Petagene

Petagene install

1. Download the binary
2. Download corpus.tar.gz from the following page: http://www.petagene.com/eval/genecodeq/ (please note that this is a 21GiB file)
3. Unpack corpus.tar.gz to some path (e.g. /var/genecodeq/)

petagene Use

Using GeneCodeq for FASTQ files:

One can compress FASTQ input files (e.g. in.fastq.gz) in the following way: > genecodeq.1.0.1.centos6 -r /var/genecodeq/hs37d5.seq -v /var/genecodeq/1kGenomeProjectT16.vcf -i in.fastq.gz -o out.fastq.gz

You'll notice from above, that this takes a compressed fastq file (i.e. in.fastq.gz) and produces another compressed fastq file (i.e. out.fastq.gz).

Using GeneCodeq for BAM files:

One needs to install samtools in order to use GeneCodeq with BAM files. The systems that your client uses should most likely have this already installed. If they don't, it can be downloaded from http://www.htslib.org/download/

Once samtools is installed, you can use GeneCodeq to compress BAM files (e.g. in.bam) in the following way: > samtools view -h in.bam | genecodeq.1.0.1.centos6 -r /var/genecodeq/hs37d5.seq -v /var/genecodeq/1kGenomeProjectT16.vcf -t SAM | samtools view -b - -o out.bam

In both cases (FASTQ or BAM), you should see a considerable difference in file sizes (e.g. in.fastq.gz vs out.fastq.gz and in.bam vs out.bam). As I said before, please share any performance numbers you gather with us.